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Materials and methods Materials HPLC-grade acetonitrile (MeCN), methanol (MeOH), ammonium bicarbonate (NH 4 HCO 3 ), formic acid (FA), ethanol, diethyl ether, 0.1 mol/L hydrochloric acid (HCl), sodium hydroxide, disodium hydrogenphosphate dodecahydrate (Na 2 HPO 4 ·12H 2 O), sodium dihydrogenphosphate dihydrate (NaH 2 PO 4 ·2H 2 O), glutathione (GSH), hydrochloric acid, dimethyl sulfoxide-d 6 (DMSO-d 6 ), CBB stain One Super, running buffer solution (10×) for SDS-PAGE, and Extra PAGE One Precast Gel 10–20% were purchased from Nacalai Tesque, Inc