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However, a number of issues cloud this principle and influence the selective advantage of efficient ROS detoxification systems

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After three washes in PBS, sections were incubated for 3 h in blocking solution (3% bovine serum albumin (BSA), 0.3% Triton X-100 in TBS) and then for 24 h at 4 °C with mouse monoclonal antibodies against tyrosine hydroxylase (TH) diluted in PBS containing 1% BSA