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General guidelines by use case: Acute injury support : 4 to 6 weeks at 250-500 mcg twice daily Gut healing protocol : 6 to 8 weeks at 250-500 mcg twice daily Joint support : 4 to 8 weeks, adjusted based on response Maintenance : Some providers prescribe shorter 2 to 4 week cycles with breaks in between After completing a cycle, most protocols call for a 2 to 4 week break before restarting