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The digested samples were then subjected to LC–MS/MS analysis using a ShimazuLC (LC-20AB pump) system coupled to a 4000 QTRAP triple-quadrupole MS instrument (AB Sciex) and a C18 column (250 mm × 2.1 mm inner diameter, 3-μm particle size

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Tym, co czyni GHK-Cu cząsteczką wyjątkową, jest rola miedzi: peptyd GHK pełni funkcję jej fizjologicznego nośnika, a dopiero kompleks z jonem Cu²⁺ łączony jest w literaturze z pełnym zakresem aktywności reparacyjnej — w odróżnieniu od wolnego peptydu GHK bez miedzi